Intermediate

Part:BBa_K133058:Design

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)

CF-multiHP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1207
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 764
    Illegal BamHI site found at 1275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

plasmid, GeneArt (Multiepitope)

References